Recombinant Protein from Trypanosoma Congolense a Good Diagnostic Antigen in Immunochromatographic Test

  • MochaboK. M., Suganuma K., Thu-Thuy N., Zhou M., Shin-ichiro K., Suzuki Y. and Inoue N.
Keywords: Immunochromatographic test, Nagana, Trypanosome, Vaccine

Abstract

Trypanosomes are hemo-flagellate protozoan parasites that cause disease in humans and livestock called sleeping sickness and nagana, respectively. So far, a vaccine has been elusive because of the immunodominant variant surface glycoprotein (VSG) in a phenomenon known as antigenic variation. According to amino acid domains structure, Tc38630 was assumed as a Trypanosoma congolense orthologue of the T. brucei invariant surface glycoprotein (ISG) therefore, a potential diagnostic antigen. Recombinant protein Tc38630 was successfully expressed, characterized and found to be antigenic. Among other serological tests, immunochromatographic test (ICT) has an advantage as a one-step rapid analysis, thus making it a convenient and sensitive diagnostic test .BALB/c mice were immunized with rTc38630 protein and serum was collected for IgG polyclonal antibodies purification for use as control line in ICT. The antigen-antibody reaction was detected by colloidal gold conjugated rTc38630 protein at the test line. The ICT result was found to be consistent with rTc38630 protein-based ELISA. For vaccine studies, five groups of BALB/c mice, were randomized and assigned as recombinant protein, recombinant GST, MCF homogenate, BSF homogenate and PBS and immunized respectively. They were challenged with a lethal dose of T. congolense. The recombinant protein did not protect the mice (survival analysis, P=0.09).

Author Biography

MochaboK. M., Suganuma K., Thu-Thuy N., Zhou M., Shin-ichiro K., Suzuki Y. and Inoue N.
  1. National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, 080- 8555, Obihiro, Hokkaido, Japan. Research Center for Zoonosis Control, Hokkaido University, 060-0808, Sapporo, Hokkaido, Japan.
  2. Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine and Surgery, Egerton University, P.O. Box 536-20115, Njoro, Kenya

*Corresponding author: kmochabo@gmail.com

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Published
2019-05-09
How to Cite
MochaboK. M., Suganuma K., Thu-Thuy N., Zhou M., Shin-ichiro K., Suzuki Y. and Inoue N. (2019). Recombinant Protein from Trypanosoma Congolense a Good Diagnostic Antigen in Immunochromatographic Test. Egerton Journal of Science and Technology, 16(1-139). Retrieved from https://eujournal.egerton.ac.ke/index.php/EJ/article/view/28
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